{"id":3887,"date":"2025-02-25T10:15:34","date_gmt":"2025-02-25T10:15:34","guid":{"rendered":"https:\/\/lipidomics.at\/lipidomics-forum\/?p=3887"},"modified":"2025-05-21T09:02:45","modified_gmt":"2025-05-21T09:02:45","slug":"workshop-lifs-tools","status":"publish","type":"post","link":"https:\/\/lipidomics.at\/lipidomics-forum\/2025\/02\/workshop-lifs-tools","title":{"rendered":"Workshop 1 – LIFS Tools"},"content":{"rendered":"\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t

Workshop 1<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t

LIFS Tools<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t

\nDate & Time<\/b> Sunday, September 14th 2025\n
\nContents<\/b>\nIn the first part of the course, we will work through an example of targeted LC-MS lipidomics with LipidCreator and Skyline. In the second part of the course, we will provide insights into using LipidXplorer to identify and quantify lipids from shotgun lipidomics. The course will consist of a short theory and background overview of the employed programs complemented by applying the tools to provided data sets. The third part of this course will work through a typical use-case of downstream data processing of shotgun lipidomics data following MS acquisition with LipidXplorer. We will inspect, check, and normalize the data and calculate absolute quantities using internal class-specific standards with lxPostman. We will then perform a qualitative and quantitative comparison of the lipidomes using LipidSpace.\n
\nLearning goals<\/b>\nParticipants will be able to understand and explain the targeted shotgun MS and LC-MS workflows for lipidomics. They will learn the fundamentals of the software tools used and how to choose parameters for them. They will learn to understand and interpret the results of each pipeline step.\n
\nPrerequisites<\/b>\nBasic knowledge of lipidomics, analytical workflows in lipidomics, and basic familiarity with web-based and desktop applications. Please note that you may need to install software on your computer to fully participate in all exercises requiring proper rights. The workshop will be a mix of small lecture segments and hands-on exercises. The trainers will be available for questions and assistance during the workshop. We will use Zoom to host the workshop. To participate, please install the Zoom client software on your computer.\n
\nKeywords<\/b>\nLipidomics, Shotgun, Targeted Analysis, LC-MSn, Reporting\n
\nTools<\/b>\nLipidXplorer, LipidCreator, Skyline, Goslin, lxPostman, LipidSpace, Reporting Checklist\n<\/div>\n\t\t\t\n\t\t\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t
Robert Ahrends (Univie, LIFS), Cristina Coman (Univie, LIFS), Dominik Kopczynski (Univie, LIFS), Nils Hoffmann (FZ J\u00fclich, LIFS), Dominik Schwudke (FZ Borstel, LIFS), Nina Troppmair (Univie, LIFS)<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t
\n\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\"\"\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\"\"\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\"Troppmair\"\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t
\n\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\"\"\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\"Dominik\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\"Coman-1-720x900\"\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\t\t\t\t
\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t<\/i>\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\tAccess Conference Agenda<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\t\t
\n\t\t\t
<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

Workshop 1 LIFS Tools Date & Time Sunday, September 14th 2025 Contents In the first part of the course, we will work through an example of targeted LC-MS lipidomics with LipidCreator and Skyline. In the second part of the course, we will provide insights into using LipidXplorer to identify and […]<\/p>\n","protected":false},"author":6,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"elementor_header_footer","format":"standard","meta":{"footnotes":""},"categories":[6],"tags":[],"class_list":["post-3887","post","type-post","status-publish","format-standard","hentry","category-keynote"],"lang":false,"translations":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"https:\/\/lipidomics.at\/lipidomics-forum\/wp-json\/wp\/v2\/posts\/3887","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lipidomics.at\/lipidomics-forum\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lipidomics.at\/lipidomics-forum\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lipidomics.at\/lipidomics-forum\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/lipidomics.at\/lipidomics-forum\/wp-json\/wp\/v2\/comments?post=3887"}],"version-history":[{"count":28,"href":"https:\/\/lipidomics.at\/lipidomics-forum\/wp-json\/wp\/v2\/posts\/3887\/revisions"}],"predecessor-version":[{"id":4530,"href":"https:\/\/lipidomics.at\/lipidomics-forum\/wp-json\/wp\/v2\/posts\/3887\/revisions\/4530"}],"wp:attachment":[{"href":"https:\/\/lipidomics.at\/lipidomics-forum\/wp-json\/wp\/v2\/media?parent=3887"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lipidomics.at\/lipidomics-forum\/wp-json\/wp\/v2\/categories?post=3887"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lipidomics.at\/lipidomics-forum\/wp-json\/wp\/v2\/tags?post=3887"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}